To analyze the neuroblastoma CRC in MYCN-driven tumors with disrupted lmo1 expression, we dissected the EGFP-fluorescent tumors from juvenile zebrafish, mechanically prepared a single-cell suspension, sorted the EGFP+ cells before RNA extraction, and conducted RNA-Seq to compare the transcriptomes of zebrafish MYCN-driven neuroblastomas with the GATA/GATA, TATA/TATA and lmo1–/– lines. The gene discussed is QRSL1; the disease is neuroblastoma.