Lipids from brains isolated from WT siblings and psap+63/+63 or psap−14/−14 zebrafish at the end stage of disease (∼4 mpf), characterized by significantly impaired locomotion and/or cachexia, were extracted using the Bligh–Dyer method (Bligh and Dyer, 1959) for liquid chromatography–mass spectrometry (LC–MS) analysis. The gene discussed is PSAP; the disease is Cachexia.