In addition, using systemic in vivo DNA labeling with 5-ethynyl-2′-deoxyuridine (EdU), we found EdU were incorporated in OASL+ MG5 and CH25H+ MG6 subclusters in the ischemic stroke brain 3 days after stroke (Fig. 3E, F, Additional file 2: Fig. S4B). The gene discussed is CH25H; the disease is ischemic stroke.