To explore the detailed transcriptional changes of the highly heterogenous microglia after ischemic stroke, we reclassified microglia (Additional file 2: Fig. S2A) and found seven distinct microglia subclusters, MG0–MG6 (Fig. 1A), which all expressed canonical microglia genes, including P2ry12, Hexb, Sparc, and Olfml3 (Fig. 1B). The gene discussed is HEXB; the disease is ischemic stroke.