Finally, we constructed lentivirus inducted stable overexpression of CCL20 in U14 (OE-CCL20), and verified the successful overexpression by qRT-PCR, western blot, flow cytometry and IHC (Additional file 1: Figure S5).Then subcutaneous U14 tumor models were established in C57BL/6 mouse. This evidence concerns the gene CCL20 and neoplasm.