*3 biomarkers: CXCL4, S100A8, and S100A9 showed increased serum levels/tissue overexpression in glioblastoma compared with control*CXCL4 upregulation shows early tumor growth*S100A8 and S100A9 are overexpressed in various types of cancers including the current analysis in GBM. Linked with tumorigenesis. This evidence concerns the gene PF4 and neoplasm.