*606 cell surface proteins (p-value <0.05) identified and quantified.*35 cell surface proteins overexpressed in GBM compared with astrocytes and NPCs*Gene expression analysis revealed 23 genes associated with GBM in accordance with glycoproteomic analysis.*19 out of 23 genes indicated correlation between mRNA expression and protein expression in GBM and astrocytes.*Known biomarkers for glioma biology: PTPRZ1, GPR56, TNC, IL13RA2, ICAM1, NCAM1, THBD, and NTRK2*Novel markers for glioma biology: found: SLC1A3, CLU, LGALS3BP, ANGPTL2, CRYAB, and ITGB8. Here, CLU is linked to central nervous system cancer.