From our work, after analysis of 4 HCC scRNA-seq datasets, we found that two of the 3-gene signature, KIF18A and EZH2 had correlation coefficient with Tprolif cells, which indicated KIF18A/EZH2/Tprolif cells axis might be a pathway in initiation and progression of HCC. This evidence concerns the gene EZH2 and hepatocellular carcinoma.