As Alpha-fetoprotein (AFP) and Golgi phosphoprotein 2 (GOLPH2) are expressed in a high level in HCC, two new oncolytic adenoviruses are developed by replacing the endogenous E1B promoter with the AFP promoter (ZD55) and the GOLPH2 promoter (GD55), respectively. The gene discussed is AFP; the disease is hepatocellular carcinoma.