Studies utilising B16-F10 murine melanoma cells cultured on poly-HEMA (an ultra-low attachment coating) demonstrate increased expression of p-FAK, alongside increased expression of downstream molecule RhoA, and increased activating phosphorylation of Akt (p-Akt) and ERK1/2 (p-ERK1/2) when compared to the Swiss 3T3 anoikis sensitive cell line used as a control (Goundiam et al., 2010). This evidence concerns the gene AKT1 and melanoma.