Considering that the fasting blood glucose of insulin resistant C57BL/6J mice may be >240 mg/dL [40], the overall implication of the metabolic outcomes of the HFS-fed C57BL/6J mice in our study, especially as it relates to glucose metabolism, suggest that these animals are likely in the early stages of developing obesity-induced insulin resistance. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.