To further validate that FUBP1 mediates the chemoresistance of osteosarcoma cells through AA metabolic pathway, PGE2 and leukotriene B4 (LTB4) were detected in the supernatants of tumor cells, and both showed elevated expression in cotransfected cells compared to FUBP1‐knockdown osteosarcoma cells (Figure 7E). This evidence concerns the gene FUBP1 and neoplasm.