The study showed that the balance within the tumor was skewed towards a pro-inflammatory phenotype in pHIFU + ICI-treated mice caused by an acute infiltration of CD8+ TILs and CD8+ IFNγ+ TILs, raising the ratio of CD8+ IFNγ+ TILs to regulatory T Cells, MDSCs and CD4+ T Cells while retaining the levels of regulatory T Cells and MDSCs at the levels seen in control mice. The gene discussed is CD4; the disease is neoplasm.