It was found that CRT-NP plus FUS (CFUS) upregulated the CRT expression, expanded the population of melanoma TRP-2 specific functional CD4+ and CD8+ T Cells and tumor-suppressing M1 phenotype macrophages, and increased the expression of PD-1 and PD-L1 in T Cells. The gene discussed is PDCD1; the disease is neoplasm.