CD8A and neoplasm: Cytofluorimetric analysis of the tumor immune infiltrates showed that inclusion of Ad-9D9 to the combination of GAd-62nag and anti-PD-1 induces the highest increase in CD8 T cells frequency and the deepest depletion of intratumoral Treg cells, compared with the GAd-62nag vaccine alone and the dual combination GAd-62nag + anti-PD-1 (Figures 4A, B).