TNF-α immunolabeling was greater in granulomas of calves observed in the cytoplasm of epithelioid MΦs, MGCs, and some fibroblasts found in cellular areas of granulomas and extracellularly, with greater intensity around necrotic sections and less intensity in the periphery of the lesion (Figures 4I,L). The gene discussed is TNF; the disease is Granuloma.