ESR1 and neoplasm: Median expression levels of signatures reflecting antigen presenting machinery (APM), IFN-γ signature, inflammatory chemokines and tumor-inflammation score (TIS) were all highest in breast tumors with no ER expression, not significantly different from the groups with low- or intermediate-positive ER expression and statistically significantly higher when comparing to the ER51-99% or ER100% groups (Fig. 2e–h).