Material and Methods: RNA expression and clinical data of The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) were acquired, and we employed couple of bioinformatics methods to investigate the potential biological and immunological role DLL3, including pan-cancer expression, survival analysis, GSVA and it’s correlation with immune infiltration scores, tumor mutation burden, tumor microsatellite instability. This evidence concerns the gene DLL3 and neoplasm.