KM survival analysis demonstrated that there were statistical significances in 23 types of cancer (Supplementary File 1) and the 6 types with dominant significances were shown in (Figure 2B), that is, among patients with LGG (p = 2.44e-10) and LAML (p = 2.49e-04), those with high levels of DLL3 had longer survival times, while in patients with KIRP (p = 1.38e-13), SKCM (p = 1.31e-05), LIHC (p = 7.02e-05) and UCEC (p = 2.40e-04), high DLL3 expression was associated with poor OS. This evidence concerns the gene DLL3 and cancer.