Figure 6a,b show the SERS spectra of (red) the tumor site and (blue) the liver site obtained using a 785 nm laser as an excitation light. The SERS spectra were obtained 5 h after nanoparticle injection. The power of excitation light and the Raman signal integration time were 20 mW and 2 s, respectively. The Raman reporter molecule was malachite green. SERS signals in the tumor region were significantly enhanced for the targeted nanoparticles, indicating that the scFv-conjugated AuNPs were able to detect EGFR-positive tumors in vivo [108]. The gene discussed is EGFR; the disease is neoplasm.