After the addition of bovine Lf to the culture medium, the protein was detected in the cytoplasm of HT29 cells [36], HeLa cells [37], MCF-7 cells, primary bovine mammary epithelial cells [38], mouse L929 fibroblasts, and mouse GL-261 glioma cells [39]; nuclear localization of Lf was observed only in Caco-2 cells [36]. This evidence concerns the gene LTF and central nervous system cancer.