Firstly, N2A cells from murine neuroblastoma were divided into two groups and then co-transfected with pGL4.10-Nlrp6 promotor(fLuc) and pGL4.74(rLuc), respectively, together with NPAS4 overexpression plasmids (pIRES2-Npas4-Flag) or empty vector plasmids (EV-Ctrl). Here, NLRP6 is linked to neuroblastoma.