CASP3 and neoplasm: Accordingly, Western blot analysis showed that a significant 4.64-fold increase (p = 0.001) in the expression of cleaved caspase-3 was induced after irradiation of the whole tumor while irradiation of half of the tumor volume only produced a 2.33-fold increase, which was not significant compared to unirradiated tumors (Figure 3H,I).