Here, using an animal model of transient cerebral ischemia and a proteomic approach with 2D DIGE combined with MALDI-TOF MS, and protein validation by Western blotting, we demonstrate the specific association of different DRP2 isoforms with 4E-BP2 and eIF4E proteins, with significant differences between resistant and vulnerable brain regions in response to ischemia reperfusion. The gene discussed is DRP2; the disease is transient ischemic attack.