Healthy urine induced a 2.5-fold increase in the TP53 mRNA expression and a 1.7-fold increase in the TP51 expression, while AKI stage 2/3 urine induced an upregulation of the TP53 mRNA by 3.5-fold and the SIRT1 mRNA by 4.2-fold. The gene discussed is TP53; the disease is acute kidney injury.