To test the hypothesis that the chemokines found to be upregulated in the AKI stage 2/3 urine have a direct influence on the expression levels of TP53 and SIRT1, we incubated our recently published immortalized podocyte cell line UM51 Htert [27] with a medium composed of 10% of the patient urine 72 h post-surgery for 120 h. This evidence concerns the gene SIRT1 and acute kidney injury.