While the SIRT1 expression was not detectable in the podocytes under control conditions, it significantly increased in the podocytes incubated with healthy urine to 600% (p < 0.01) and was even found to be further elevated when the podocytes were incubated with AKI stage 2/3 urine to 1051% (p < 0.01). The gene discussed is SIRT1; the disease is acute kidney injury.