Next, to study the effects of hyperglycaemia and hyperlipidaemia and its effects on the expression levels of PIMT and key markers of pancreatic β-cells in an in vivo type 2 diabetic animal model, we isolated islets from mice fed with a high fat diet (HFD) for a period of 16 weeks. The gene discussed is TGS1; the disease is type 2 diabetes mellitus.