Immunohistochemistry (IHC) staining for BTBD16, EpCAM, FOXM1, AGXT and JMJD1C was carried out in normal and tumor thyroid tissue samples (three patient biopsies available), belonging to two NMTC families: NMTC_1 (Figure 3A) and NMTC_4 (Figure 3B). The gene discussed is BTBD16; the disease is neoplasm.