Since the ectopic overexpression of NRP-1 in BT-474 breast cancer cells was associated with the upregulation of tenascin C (TN-C) [9], we next explored this relationship in MDA -231, NRP-1 KO, and NRP-1 Rescue cells using immunofluorescence staining (Figure 2, upper left). Here, TNC is linked to breast carcinoma.