In accordance with these studies, we evaluated the impact of berberine on cell growth, migration, invasion, apoptosis, and expression level of certain genes (PI3K, PTEN, AQP1, AQP3 and AQP5) in HT-29, SW-480 and HCT-116 colon cancer cell lines. The gene discussed is AQP1; the disease is malignant colon neoplasm.