In this regard, the bioavailability and anti-tumor activity of the anti-PD-L1 antibody are enhanced when co-administered with hyaluronidase-coupled HEK 293 cell-derived EVs, due to the degradation of hyaluronan in the TME in a breast cancer model, inducing a memory anti-tumor immune response dependent on Tc cells [164]. This evidence concerns the gene CD274 and breast carcinoma.