Mangiferin suppresses MMP-9 activity in human astroglioma cells at the promoter, mRNA, and protein levels through the inhibition of nuclear factor-κB (NF-κB) and AP-1 binding to the MMP-9 promoter and the down-regulated phosphorylation of Akt and MAP kinases, two upstream signaling molecules of MMP-9. This evidence concerns the gene MMP9 and astrocytic tumor.