Nevertheless, a limitation of our study is that it did not assess the nuclear content of CREB/ICER, to confirm the real CRE-activated transcriptional activity, pointing out that sequestration of CREB in granules of GVD in the hippocampal neurons of AD brains was described as being accompanied by a reduction in CREB nuclear content [70]. Here, CREB1 is linked to Alzheimer disease.