In comparisons of the baseline characteristics between the MSAF less than 1% and MSAF greater than or equal to 1% subgroups, a low MSAF (<1%) was associated with favorable prognostic factors, such as younger ages, fewer current smokers, more patients with a PD-L1 positive status, lower number of target lesions, and smaller tumor sizes, which may suggest a lower tumor burden. This evidence concerns the gene CD274 and neoplasm.