Therefore, a lung metastasis model of liver cancer in nude mice was established by injecting SQSTM1 WT HepG2 cells or SQSTM1 KO HepG2 cells into the tail vein to evaluate and verify the effects of SQSTM1/p62 on the migration and invasion of HCC cells in vivo. The gene discussed is SQSTM1; the disease is hepatocellular carcinoma.