To further confirm the effect of SQSTM1/p62 on the migration and invasion of HCC cells, we used the SQSTM1/p62 knockout cell lines (SQSTM1 KO HepG2 cells and SQSTM1 KO2 HepG2 cells) and found that after SQSTM1/p62 knockdown, the expression level of Keap1 was significantly increased (Figure 4A and Figure A3A), while the expression level of Nrf2 was significantly decreased (Figure 4B and Figure A3B). Here, KEAP1 is linked to hepatocellular carcinoma.