A representative IHC expression of HIF-1α in normal kidney tissue and adjoining ccRCC tumour is presented in Figure 2A. A comparison of the in-house patient’s normal kidney tissue by IHC showed the expression of HIF-1α to be moderate in the normal kidney tubules, while no staining in the primary tumour was observed (Figure 2A). This evidence concerns the gene HIF1A and nonpapillary renal cell carcinoma.