TWIST1 and nonpapillary renal cell carcinoma: In another study, patients diagnosed with ccRCC showed higher expression of ZEB2 and TWIST proteins in low-grade compared to high-grade tumours, which may imply that the process of EMT is initiated at the early stages of tumour development, suggesting that the evaluation of EMT-associated proteins, may be useful for the assessment of the metastatic potential of tumours in patients [14].