Overexpression of activated αVβ3 mutant in the MDA-MB-435 breast cancer cell line increased brain metastasis by way of VEGF upregulation and increased angiogenesis [168], whereas an anti-integrin αV monoclonal antibody reduced brain metastasis of MDA-MB-231-breast cancer cells overexpressing the HER2 oncogene [169]. This evidence concerns the gene ERBB2 and breast cancer.