Importantly, the unmethylated liver cancer cell lines at the NSUN7 5’-end CpG island (HEP3B2-1-7, SNU-475 and SNU-387) expressed NSUN7 RNA, determined by quantitative real-time PCR, furthermore the NSUN7-hypermethylated cells from SNU-423, SNU-398 and HUH-7 showed lack or minimal expression of the transcript (Fig. 1E). Here, NSUN7 is linked to liver cancer.