In summary, we performed a comprehensive decipherment of the spatial structure of intratumoral CD8+ T cells in NSCLC patients with LNM based on mIF images, including special spatial distribution of CD8+ T-cell functional subsets and spatial interplay between CD8+ T cells and their neighboring cells, which revealed the complexity of TME with significant implications for facilitating precise immunotherapy. Here, CD8A is linked to non-small cell lung carcinoma.