Considering the expression level of FOXK1 in ESCC cell lines, the pcDNA3.1-FOXK1 plasmid was used to overexpress FOXK1 in KYSE170 and TE1 cells, and siRNAs (si-FOXK1-1 and si-FOXK1-2) were used to knockdown FOXK1 in KYSE150 and TE1 cells. The gene discussed is FOXK1; the disease is esophageal squamous cell carcinoma.