TP53 and lung cancer: In that study, the authors used both a conditional activated Cas9 transgenic mouse line and an AAV vector (AAV-KPL) that carried the luciferase reporter and Cre in addition to gRNAs (targeting Tp53, Lkb1, and Kras) and the HDR donor sequence for KrasG12D to model lung cancer by causing indel mutations of tumor suppressor genes Tp53 and Lkb1 and missense mutation of Kras, but the resulting tumors only showed a negligible level of KrasG12D missense mutations.