To better define whether there is a requirement for LAG3 either during the maintenance phase or at the time of priming for E. multilocularis infection-induced CD4+ T helper cell polarization, we modified our adoptive transfer protocol to establish infection in either CD4+ T-cell LAG3-KO mice or WT CD45.1 recipients that had been infected 30 days or one day before E. multilocularis infection (Figs 5A and 6A). This evidence concerns the gene CD4 and infection.