To this end, axotomized cultures were fixed 0.5, 1, 3 or 12 h after live-cell monitoring, and total Tau levels or phosphorylation at the AT8 motif, which is often hyperphosphorylated in tauopathies (Arendt et al. 2016), was assessed with immunostaining. This evidence concerns the gene MAPT and tauopathy.