In addition to a propensity for liver damage, HFD-fed UGDH-deficient mice, in comparison to Ugdhf/f mice on the same diet, also exhibited greater levels of lipid accumulation and steatosis in the liver, as indicated by H&E and Oil red O staining (Supplementary Fig. 5e), as well as by levels of hepatic TG and TC (Supplementary Fig. 5f). Here, UGDH is linked to steatosis.