GH1 and chronic kidney disease: Moreover, to examine the effects of the optogenetic inhibition of SFO neurons projecting to the PVN, AAV2/retro-CaMKIIα-eNpHR 3.0-EYFP-WPRE-hGH pA was injected into the PVN of CKD or HF mice, and the cell bodies of SFO neurons with eNpHR3.0 were exposed to yellow light (Fig. 6a, b).