To confirm the regulatory roles of these cytokine(s) in the induction of NOS2 and/or COX2 expressions, MDA-MB231 (MB231) breast cancer cells were stimulated with IFNγ in the presence and absence of TNFα, IL1β, IL17, and the TLR4 agonist lipopolysaccharide (LPS). This evidence concerns the gene NOS2 and breast cancer.