Given the predictive power of CD8+ T cell spatial localization [22], we observed abundant stroma-restricted CD8+ T cells (Fig. 4C) with increased IFNγ expression (Fig. 4D) in regions proximal to elevated tumor NOS2 expression, indicating a potential association between CD8+ T cells, IFNγ, and NOS2 regulation (Fig. 4C, D). This evidence concerns the gene NOS2 and neoplasm.