Our analysis showed that the overall pooled mutation frequencies of these major ALS-related genes are 2.2% for SOD1, 2.1% for C9orf72, 1.7% for ATXN2, 1.7% for FUS, 0.8% for TARDBP, 0.6% for VCP, 0.6% for SQSTM1 and 0.6% for UBQLN2. Subgroup analysis of whether there was a family history of ALS, and several conclusions could be drawn from the results. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.