In conclusion, we identified FDX1 and SLC31A1 as signature genes for cuproposis in DCM, and cardiomyocytes exhibiting higher cuproposis scores, B-cell naive, Eosinophils, NK cells activated and T-cell CD4 memory resting associated with immune infiltration of cuproposis in DCM, and identified potential natural compounds rutin and Polydatin. This evidence concerns the gene CD4 and familial dilated cardiomyopathy.