GCG and Hypoglycemia: On the other hand, a trebling of plasma alanine concentrations (from ~300 to ~870 μmol/L) had no effect on plasma glucagon levels in healthy individuals (63), suggesting that the small differences in alanine observed between groups (311 vs. 218 μmol/L before hypoglycemia in FED and FAST, respectively, and 210 and 155 μmol/L during hypoglycemia, respectively; Table 2), would not be expected to enhance glucagon secretion.