Supplying SAM (Figure 2, I and J) or 3PG (Supplemental Figure 2, J and K) to PKO but not PKO/SUV-KO cells reduced the growth and migration abilities of HCC cells, indicating that SUV39H1 is the primary methyltransferase involved in mediating H3K9me3 modification in these hepatoma cells. This evidence concerns the gene SUV39H1 and hepatocellular carcinoma.