Consistently, we found that IDHmt glioma cells contained significantly less Filipin+/Nestin+ volumes than IDHwt counterparts, indicating a reduced intracellular cholesterol level in IDHmt tumor parenchyma (Figure 3C,D, IDHwt: IDHmt = 0.52: 0.19, p = 0.0012). This evidence concerns the gene NES and glioma.