Moreover, optimized 5A2‐DOT‐X LNPs prepared by microfluidic mixing effectively delivered RNPs to restore dystrophin expression in Duchenne's muscular dystrophy (DMD) mice and considerably decreased serum proprotein convertase subtilisin/kexin type 9 (PCSK9) levels in C57BL/6 mice.[82]. This evidence concerns the gene PCSK9 and Duchenne muscular dystrophy.