Parackova et al. [56] determined that NETs can be used in the development of diabetes mellitus by stimulating with PMA NETs from type 1 diabetes mellitus (T1D) patients and NETs fragments isolated from peripheral blood of healthy control donors, cleavage of NETs with a restriction endonuclease mixture, and co-culture and further compositional analysis using fractions consisting of large DNA fragments and NETs-associated proteins, showed that the presence of NETs induces IFN-γ-producing T cells in vitro and activates T cells toward IFN-γ-producing CD4 and CD8 polarization. This evidence concerns the gene CD4 and type 1 diabetes mellitus.