PRKN and metabolic dysfunction-associated steatotic liver disease: Notably, the previous observations made in the mito-Keima mice and our own work with the liver-specific PARKIN knockout mice utilized high-fat diets that modeled the metabolic aspects of NAFLD (steatosis and insulin resistance) but not the inflammatory or fibrotic features25,26, and both were limited to single dietary durations (18 and 12 weeks, respectively for mito-Keima and LKO studies).