HGF and glioblastoma: To determine whether C-HGF had direct effects on c-MET activity in GBM cells we generated recombinant His-tagged C-HGF and found that GBM6 or HK296 GBM cells when treated with this recombinant protein (rC-HGF) resulted in significant activation of c-MET as monitored by P-Y1234-MET and P-Y1235-MET autophosphorylation (Fig. 4A).